mouse anti human cd2 monoclonal antibody  (Miltenyi Biotec)

Journal: Cell reports

Article Title: A P53-Independent DNA Damage Response Suppresses Oncogenic Proliferation and Genome Instability

doi: 10.1016/j.celrep.2020.01.020

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Mouse Anti-Human CD2 Monoclonal Antibody, Clone LT2 (1:10 for Flow) , Miltenyi Biotec , Cat# 130-091-115, RRID:AB_244321.

Techniques: Virus, Recombinant, Modification, Adhesive, Single Cell Gel Electrophoresis, TA Cloning, Sequencing, Generated, Software

Journal: PLoS ONE

Article Title: A Simple Detection Method for Low-Affinity Membrane Protein Interactions by Baculoviral Display

doi: 10.1371/journal.pone.0004024

Figure Lengend Snippet: Ten micrograms of each of the BV samples expressing CD2 or its ligand CD58 (A), CD40 or CD40L (B), and GITR or GITRL (C) were loaded in each lane. The blotted membranes were immuno-stained with either anti-FLAG or anti-HA antibodies according to the attached tag as described in . The positions of the molecular mass marker proteins are indicated on the left .

Article Snippet: Monoclonal mouse anti-human CD2 (clone RPA-2.10, unconjugated or biotinylated), anti-human CD58 (clone 1C3 (AICD58.6)), rat anti-mouse CD40 (clone 3/23), hamster anti-mouse CD40 (clone HM40-3), and biotinylated hamster anti-mouse CD40L (clone MR1) antibodies were from BD PharMingen.

Techniques: Expressing, Staining, Marker

Journal: PLoS ONE

Article Title: A Simple Detection Method for Low-Affinity Membrane Protein Interactions by Baculoviral Display

doi: 10.1371/journal.pone.0004024

Figure Lengend Snippet: (A) Schematic view of the ELISA system. Details are described in the text. X and Y correspond to CD2 and CD58 (or vice versa for C) respectively. (B) Left panel; binding of CD58-BV to immobilized CD2-BV (filled circles) or wild-type BV (open circles). Right panel: Blocking of CD58-BV binding to the plate-bound CD2-BV by pre-incubation of wells with an anti-CD2 antibody (filled circles) or control mouse IgG1 (open circles). (C) Left panel: binding of CD2-BV to the immobilized CD58-BV (filled circles) or wild-type BV (open circles). Right panel: blocking of CD2-BV binding of the plate-bound CD58-BV by pre-incubation of wells with an anti-CD58 antibody (filled circles) or control mouse IgG2a (open circles). Each well was coated with 0.5 µg of BV. Each data point represents detection in triplicate (error bar, 1 s.d.).

Article Snippet: Monoclonal mouse anti-human CD2 (clone RPA-2.10, unconjugated or biotinylated), anti-human CD58 (clone 1C3 (AICD58.6)), rat anti-mouse CD40 (clone 3/23), hamster anti-mouse CD40 (clone HM40-3), and biotinylated hamster anti-mouse CD40L (clone MR1) antibodies were from BD PharMingen.

Techniques: Enzyme-linked Immunosorbent Assay, Binding Assay, Blocking Assay, Incubation

Journal: PLoS ONE

Article Title: A Simple Detection Method for Low-Affinity Membrane Protein Interactions by Baculoviral Display

doi: 10.1371/journal.pone.0004024

Figure Lengend Snippet: (A) Schematic view. X corresponds to CD2 (for B), CD40 (for C), or GITR (for D). Y corresponds to CD58 (for B), CD40L (for C), or GITRL (for D). (B) CD58-displaying BV binds to CD2-positive (top), but not to CD2-negative Jurkat cells (bottom). Left: binding of an anti-CD2 monoclonal antibody (clone RPA-2.10) plus a PE-anti mouse Ig-κ chain antibody. Right: binding of CD58-displaying BV plus an anti-gp64 antibody (clone A0505A) and a PE-anti mouse Ig-κ chain antibody. The thin line indicates cells incubated with the 2nd antibody only. (C) CD40-dependent binding of CD40L-displaying BV to mouse splenic B cells. BALB/c mouse splenocytes were incubated with CD40L-displaying BV and a biotinylated anti-gp64 monoclonal antibody (clone B8147A), and then were stained with FITC-labeled streptavidin and a PE-labeled anti-B220 antibody. CD40L-BV binding to the B cells (shown in the center lower panel) was blocked by pre-incubation with an anti-mouse CD40 monoclonal antibody (clone HM40-3) (the right upper panel) but not by control hamster IgG (right lower panel). (D) Binding of GITRL-displaying BV to GITR-positive cells. GITR-expressing mouse T cell hybridoma 18.3.5 cells were incubated with GITRL-displaying BV (right) or wild-type BV (left). Binding of BV was detected with a biotinylated anti-gp64 monoclonal antibody B8147A and PE-streptavidin. The thin line indicates cells incubated without BV.

Article Snippet: Monoclonal mouse anti-human CD2 (clone RPA-2.10, unconjugated or biotinylated), anti-human CD58 (clone 1C3 (AICD58.6)), rat anti-mouse CD40 (clone 3/23), hamster anti-mouse CD40 (clone HM40-3), and biotinylated hamster anti-mouse CD40L (clone MR1) antibodies were from BD PharMingen.

Techniques: Binding Assay, Incubation, Staining, Labeling, Expressing

Journal: PLoS ONE

Article Title: A Simple Detection Method for Low-Affinity Membrane Protein Interactions by Baculoviral Display

doi: 10.1371/journal.pone.0004024

Figure Lengend Snippet: (A) Enrichment of human CD2-positive cells from BaF/3 cells transfected with a human T cell cDNA library. The staining of cells with an FITC-labeled anti humanCD2 monoclonal antibody is shown. The thin line indicates cells incubated without anti-CD2 antibody. (B) Binding of anti-human CD2 antibody and CD58-displaying BV to BaF/3 cells isolated by magnetic sorting with CD58-BV. After the 3rd magnetic sorting and subcloning, cells were stained with an FITC-labeled anti-human CD2 monoclonal antibody and CD58-BV plus biotinylated anti-gp64 antibody and PE-streptavidin. Staining of a representative of three clones is shown.

Article Snippet: Monoclonal mouse anti-human CD2 (clone RPA-2.10, unconjugated or biotinylated), anti-human CD58 (clone 1C3 (AICD58.6)), rat anti-mouse CD40 (clone 3/23), hamster anti-mouse CD40 (clone HM40-3), and biotinylated hamster anti-mouse CD40L (clone MR1) antibodies were from BD PharMingen.

Techniques: Transfection, cDNA Library Assay, Staining, Labeling, Incubation, Binding Assay, Isolation, Subcloning, Clone Assay

Journal: STAR Protocols

Article Title: A protocol for generating germ-free Heligmosomoides polygyrus bakeri larvae for gnotobiotic helminth infection studies

doi: 10.1016/j.xpro.2021.100946

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Article Snippet: Mouse monoclonal anti-human CD2-PE (RPA-2.10; 1:50) , BD Biosciences , 555327.

Techniques: Recombinant, Staining, Spectrophotometry, Hood, Microscopy, Sampling

Journal: eLife

Article Title: mRNA vaccine-induced T cells respond identically to SARS-CoV-2 variants of concern but differ in longevity and homing properties depending on prior infection status

doi: 10.7554/eLife.72619

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Article Snippet: Antibody , CD2(mouse monoclonal) , Fluidigm , Cat#3151003B , (1 μg/100 μl).

Techniques:

Journal: PLoS ONE

Article Title: The Tumor Targeted Superantigen ABR-217620 Selectively Engages TRBV7-9 and Exploits TCR-pMHC Affinity Mimicry in Mediating T Cell Cytotoxicity

doi: 10.1371/journal.pone.0079082

Figure Lengend Snippet: A) Analysis of flow cytometry sorted SEA activated T cells and B) cytotoxicity (SADCC) measured against Caki-2 cells with (filled) or without (open) ABR-217620 using a standard 4 hr 51 Cr-release assay of unsorted (squares) and sorted (triangles) cells binding the [ABR-217620-biotin/SA-PE]-complex and anti-CD3. C) Activation of NFκB-luciferace reporter gene in J.RT3-T3-5 cells expressing TRBV7-9 by Caki-2 cells and different concentrations of ABR-217620 (squares) or SEA/E-120 (triangles). Activation of the NFκB-luciferace reporter without ABR-217620/SEA/E-120 was equal to the defined 0-value in the graph. D) Activation of NFκB-luciferace reporter gene in J.RT3-T3-5 cells expressing TRBV7-9 by 5T4- and anti-CD2-coated (squares) or control-coated (triangles) beads and different concentrations of ABR-217620. Activation of the NFκB-luciferace reporter without ABR-217620 was equal to the defined 0-value in the graph.

Article Snippet: Mouse monoclonal anti-human 5T4 (Mab5T4-H8) was produced at Active Biotech and the mouse monoclonal anti-human CD2 purchased from Abcam, UK.

Techniques: Flow Cytometry, Release Assay, Binding Assay, Activation Assay, Expressing